FastQCFastQC Report
Wed 7 Dec 2016
30_71_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30_71_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42435198
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAC75400.016.62624540
TTCGCAA86100.014.81124439
CGACGAT26000.014.7200561
CGCAACA90400.014.01118641
GCATTCG100450.013.29724636
CATTCGC97300.012.9952837
CTCGTAT30400.012.86917542
ACGGCTA30850.012.610352532
ATTCGCA101700.012.60307138
CGACGGA31750.012.11570815
TCTCGTA32100.012.05295941
CGACAAT26750.011.7207051
TAAGTCG29900.011.70893420
CTCGAAT47600.011.5835791
CGCATAT7300.011.5518441
ACACGTC34050.011.16997513
TCGTATG37800.011.09318343
TATTGCG52200.011.0535567
ACGACGG35900.010.83546414
AGATCGA50700.010.7842194
Produced by FastQC (version 0.11.3)