FastQCFastQC Report
Wed 7 Dec 2016
30_72_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30_72_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37462708
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT42100.031.31782234
CCGTACG42400.031.1477233
ACCGTAC43250.030.6364432
CGTAATC44700.030.03553238
CTCGTAT44850.029.90645244
GTACGTA46300.028.71341535
TACGTAA47800.028.04142636
ACGTAAT48800.027.42229537
TCTCGTA53900.024.94967543
CACCGTA54150.024.87224431
AATCTCG58900.022.86876941
ATCTCGT65900.020.70560542
ACACGTC70050.019.4128513
GTAATCT72300.018.75126839
GATCGGA71250.017.713951
TAATCTC80950.016.93679240
ACGTCTG80250.016.91865515
CACGTCT80750.016.75954614
TCACCGT81500.016.7394430
CACACGT84400.016.37172712
Produced by FastQC (version 0.11.3)