FastQCFastQC Report
Wed 7 Dec 2016
30_73_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30_73_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40708961
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA48700.022.82698243
TATCTCG48550.022.76452841
GCGTTCG32800.018.68348743
CGTCGAT29150.018.21649412
AGTGCGT36450.017.46115940
TCGTGCG35450.017.2229867
GATCGGA65850.017.0512141
GCGTCGA32650.016.26372711
ACACGTC69850.016.25070813
TCGCAAC73500.016.0021740
CGTGCGT35200.015.5130578
ATCTCGT74250.015.203752542
TGCGAGA36400.014.47225335
TGATATA79800.014.14588136
ACGTCTG81000.014.0140915
CGCGTTT5400.013.9416721
GCGTAGC49150.013.86570910
AGGCGTA45700.013.6423188
CACGTCT83050.013.59028914
ATATATC84100.013.57620838
Produced by FastQC (version 0.11.3)