FastQCFastQC Report
Thu 8 Dec 2016
9_200214_3HS_ACTTGA_L005_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename9_200214_3HS_ACTTGA_L005_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56736777
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT69650.011.3231881
CTCGTTT83000.010.7507531
CTCGGTT81550.010.2234991
CTCGGAT104250.09.5320061
CGATAAC23600.09.2615041
CCGGAAT67400.09.1269251
CTGGAAT282500.09.0212341
CGACGAT33300.08.5937211
CTCAAAT195650.08.5802151
CTCCGAT76800.08.5672891
CTCGAAC69300.08.3889631
CGCAAAT37100.08.38161
CGCCTAT23800.08.3315831
CGGAAAT68250.08.1878681
CGAATGT62000.08.1286593
CGAGAAT60200.08.1224131
CTCGCAT44450.08.0602251
CGGGAAT60050.08.0301291
CTGAAAT247450.08.0042971
GTCGGTT58700.07.98448751
Produced by FastQC (version 0.11.3)