FastQCFastQC Report
Thu 8 Dec 2016
9_240214_3HS_GTCCGC_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename9_240214_3HS_GTCCGC_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48645745
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT59900.011.7442341
CTCGTTT74250.010.4462141
CTCCGAT68150.09.7931581
CTCGGTT66300.09.4202651
CTCAAAT166050.09.3014061
ACGTCCG33600.09.10584932
CTCGGAT89450.08.8979771
CGGAAAT56600.08.8038521
CTGGAAT229400.08.7771791
CCGGAAT55500.08.6127091
CTCAGAT210750.08.4626391
TCGAATG55350.08.4147362
CTTCGAT61500.08.285721
CAACGAT51250.08.2710541
CGAATGT51850.08.1148523
CGACAAT33750.08.0836681
CTCGAAC61550.08.0225591
CTGAAAT211800.07.97356941
CGGGGAT65100.07.8621581
CGACGAT31300.07.8519781
Produced by FastQC (version 0.11.3)