FastQCFastQC Report
Wed 7 Dec 2016
9_334_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename9_334_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36572870
Sequences flagged as poor quality0
Sequence length50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGN549660.1502917326422564No Hit
CTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAN522030.1427369522818417No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTN510990.13971832125835354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA46900.058.36736744
CTCGTAT17200.057.73192644
TAATGTG98900.027.40739844
GCGTTCG113850.024.65533343
TCGTGCG108700.024.2527897
AGTGCGT116700.023.72077240
CGTCGAT108550.023.71089412
GCGTCGA113200.022.77495811
CGTGCGT114600.022.5155228
GTGCGTT132000.022.13019641
TGCGTTC133350.021.97077642
TCTCGTA46750.021.6117943
CGGCTCG124250.021.309423
GGCTCGT122900.021.2051034
TGAAACG47550.021.111235
TGCGAGA123950.020.78558235
GTGCGTC126350.020.506919
AGGCGTA141450.019.9317998
AAGTGCG142900.019.50736439
GCGTAGC150050.019.36358610
Produced by FastQC (version 0.11.3)