FastQCFastQC Report
Wed 7 Dec 2016
9_335_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename9_335_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37099140
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT18800.094.46586644
TATCTCG62750.030.83790841
TCTCGTA63700.030.34409543
ACACGTC74900.026.11894413
GATCGGA75550.024.8291651
ATCTCGT79800.024.3302142
ACGTCTG83950.023.10024315
CACGTCT86750.022.47712514
CACACGT88650.022.18961512
GCACACG98050.020.59026111
ACTGATA100600.019.7704434
TGATATA100550.019.73749536
GATATAT108350.018.23742137
ATATATC108600.018.19543638
ATCGGAA110200.016.9751642
CTGATAT120450.016.83468635
TATATCT119500.016.75239639
TCGGAAG120050.016.0129133
TCGCAAC60550.015.10596840
ATATCTC134400.014.78281740
Produced by FastQC (version 0.11.3)