FastQCFastQC Report
Wed 7 Dec 2016
9_336_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename9_336_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42619052
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT19700.021.31771744
TCGCAAC75700.017.96046340
CGCAACA90500.015.11915541
ATTCGCA95150.014.4482338
TTCGCAA95200.014.34981939
CATTCGC95350.014.32724437
GCATTCG106800.013.82271436
CGACGGA35250.013.30453315
TCTCGTA33900.013.19909343
CGACGAT25100.012.9224821
TAAGTCG37750.012.709722520
ACGACGG39200.012.01897414
CGATAAG40950.011.87483617
CGTGGAT62000.011.3698381
AGATCGA58650.010.9812144
GACGGAC43200.010.906157516
GTAAGGT56300.010.8634928
AACGACG44300.010.781404513
GATCGAG60450.010.76143555
CGACAAT37600.010.581771
Produced by FastQC (version 0.11.3)