FastQCFastQC Report
Wed 7 Dec 2016
9_337_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename9_337_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39023320
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA25000.024.31175844
CTCGTAT34000.023.83505844
TCTCGTA42850.019.46925443
GCGTTCG32450.018.96124343
TCGTGCG30200.018.1231947
TATCTCG45650.018.08319341
CGTCGAT27550.017.40286812
TCGCAAC65400.015.73571140
GCGTCGA30550.015.69403411
AGTGCGT42200.014.94329340
CGTGCGT33300.014.7267028
CGACGGA31050.013.749413515
TGCGAGA35200.013.74739235
AGGCGTA46650.013.6566138
GCGTAGC52600.013.65179610
ATCTCGT62450.013.56923342
GTGCGTT47300.013.19344841
CGGCTCG41550.013.1762213
ACGACGG32900.013.04264714
ACACGTC64300.012.93801413
Produced by FastQC (version 0.11.3)