FastQCFastQC Report
Wed 7 Dec 2016
9_338_blood.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename9_338_blood.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36874328
Sequences flagged as poor quality0
Sequence length50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT19700.087.2461744
TATCTCG66350.030.61877641
TCTCGTA68200.029.8199143
TCACGAG75200.026.9831430
ACGAGTG73600.026.5181932
GATCGGA80300.025.095641
ACACGTC82550.024.94546913
ATCTCGT82400.024.785842
GTCACGA82850.024.4139229
CACGAGT84100.023.53919231
ACGTCTG91150.022.5687315
CACGTCT92350.022.43861214
CACACGT96200.021.65252912
AGTCACG95000.021.54125428
GATATCT98800.020.7366239
GTGGATA98050.020.41115636
GCACACG107100.019.56966411
GGATATC107100.019.39143438
ATCGGAA113100.017.9247742
TCGGAAG120650.016.8207993
Produced by FastQC (version 0.11.3)