FastQCFastQC Report
Thu 8 Dec 2016
9_60314_3HS_AGTCAA_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename9_60314_3HS_AGTCAA_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43458012
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG66300.021.8194273
CGTATGC84650.017.6350521
ATGCCGT93150.016.6407264
CCGTCTT107050.014.8126057
GCCGTCT105500.014.7743976
CTCGAAT64600.014.451521
TGCCGTC113350.013.7347435
GTATGCC109350.013.3527842
CGAATGT55300.012.5305893
TCGAATG58250.011.8187472
CTCGTTT72650.011.577611
CGTCTTC153100.010.8860928
CTCGCAT29700.010.0981281
CTCCGAT71250.09.9378051
GCTTGAA171200.09.70899216
CTCGGAT81500.09.5733551
CAACGAT55500.09.4262881
GTCGGTT48750.09.3437771
CGACGAT25500.09.2852751
ACGATTG42100.09.13818553
Produced by FastQC (version 0.11.3)