FastQCFastQC Report
Wed 26 Apr 2017
liver_A801_mmu_xQct.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_A801_mmu_xQct.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences33359139
Sequences flagged as poor quality0
Sequence length15-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT26150.017.81815142
ATCACGA29750.015.17811634
CGTATGC31550.014.93668144
GATCTCG36300.012.438766539
TCGTATG39400.012.07189243
TACCGAC34150.011.91936941
ATCTCGT39700.011.91368840
CCGTTTA17250.011.6683454
ACGATCT41450.011.00845637
TCGGTTA28200.010.9294582
CTCGGTT47650.010.8241741
GTTACCG37350.010.64091239
CTCGAAT32750.010.6272121
GATCGGA42850.010.5198671
ACGTCTG46950.010.22027415
TCGTTTT39800.010.1145962
TCACGAT45650.010.10335135
CGTCCGC30000.09.87318520
CGCGTCT11550.09.8024441
GCGTCCG31050.09.76041219
Produced by FastQC (version 0.11.3)