FastQCFastQC Report
Wed 26 Apr 2017
liver_A802_mmu_G5Hu.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_A802_mmu_G5Hu.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences38503638
Sequences flagged as poor quality0
Sequence length15-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC750840.19500494992187492TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC111400.036.0228344
TATCTCG147350.027.34392239
CTCGTAT149650.026.62913142
CGATGTA167550.025.66038534
AGATCGG84350.024.9116941
CACCGAT178500.024.38452731
TCGTATG167250.024.09891143
GATCGGA188150.024.0434781
TCACCGA183650.023.76679230
GTCACCG184800.023.57768629
ACGTCTG196200.022.59592215
ATCTCGT179900.022.25447740
ACCGATG197300.022.0976132
CCGATGT196350.021.9543233
GATGTAT196900.021.5878935
ATCGGAA215050.021.006722
GCACACG215900.020.70113411
ACACGTC217300.020.62895613
TCTCGTA198200.020.44002341
CGTCTGA217100.020.42382216
Produced by FastQC (version 0.11.3)