FastQCFastQC Report
Wed 26 Apr 2017
liver_A982_mmu_WFj0.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_A982_mmu_WFj0.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences29584457
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT423510.14315287246948624No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT358790.12127652030253588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA84400.019.6451852
ATTGGAC125550.017.8754486
CACGATC100700.016.9728131
CGATCAC100650.016.696843
AATTGGA145700.016.02075
GAATTGG148500.015.7897184
CTGGAAT251850.014.4008681
GAATGCA150750.014.27781232
ATTAACG67450.014.13497420
GGAATTG182550.014.0328383
CTCGAAT33450.013.8130421
ATACGTC83000.013.45304131
GATGGAA168850.013.29948428
GTGAGAC165300.013.29440619
CATACGT91750.013.20523930
TACGTCA85650.013.08684232
TTGGACT174400.012.8565627
TCGGTTA9550.012.84474852
AGATCAC181600.012.74223738
AATGCAG169700.012.73838433
Produced by FastQC (version 0.11.3)