FastQCFastQC Report
Wed 26 Apr 2017
liver_A983_mmu_ZPjq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_A983_mmu_ZPjq.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences35393946
Sequences flagged as poor quality0
Sequence length15-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA370360.10463936403135159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG50950.030.886439
CTCGTAT52750.029.3940742
CGTATGC56050.027.78392644
CGATGTA62150.027.04256434
CACCGAT71300.024.12894231
TCACCGA73750.023.35391230
GATCGGA73050.023.3093511
GTCACCG74200.023.17455129
ATCTCGT68750.022.74792140
ACGTCTG78100.021.68991915
TCGTATG72800.021.68926843
ACCGATG84050.020.68493732
CCGATGT85800.019.4864533
TCTCGTA83550.019.01747541
ACACGTC90900.018.90771713
CGTCTGA91100.018.53598416
GCACACG93450.018.42699211
ACGATCA89450.018.1228162
GATGTAT94150.018.11649935
ATCGGAA96000.017.715742
Produced by FastQC (version 0.11.3)