FastQCFastQC Report
Wed 26 Apr 2017
liver_A984_mmu_9K0y.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_A984_mmu_9K0y.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences23924007
Sequences flagged as poor quality0
Sequence length15-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT31550.020.35721242
ATCACGA33650.019.61285834
ATCTCGT36100.017.97715640
CGTATGC39700.017.62514144
TCGTATG39600.017.47960343
ACGATCT38050.016.96203837
GATCGGA39950.016.4007851
GATCTCG39400.016.39921639
ACACGTC48800.013.91179513
ACGTCTG48900.013.75222115
CATCACG50050.013.52532533
CGATCTC49450.013.29441338
ATCGGAA49750.013.2553212
TCACGAT51650.012.9366435
TCTCGTA51200.012.8351741
GCACACG52350.012.8114811
CTCGTTT27950.012.4418511
ATATCGT10100.012.37975744
CACGATC55000.012.24259136
CTCGAAC27600.012.2154931
Produced by FastQC (version 0.11.3)