FastQCFastQC Report
Wed 26 Apr 2017
liver_B320_mmu_v8LC.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_B320_mmu_v8LC.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences32679107
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT42350.012.6598951
CTTAGAT71650.012.1150981
ACGTTAC19000.012.04035733
CATACGT37650.011.6684330
TATTCGA19650.011.64819518
TCTTCGT91600.011.4890144
ACCCGTA11150.011.41509531
CTCGGTT38550.011.3139021
GGACATA82200.011.2429821
CTCTTCG92750.011.21382943
GACATAT82500.011.10942422
CTAGCCT107850.010.9879841
ATACGTT21250.010.92833231
GTATTCG24650.010.76048117
CTCAGCT287250.010.69523051
CGTATAG9400.010.59098219
TATATCG13150.010.32151416
AGCTTTA117950.010.2445164
CTGGAAT208950.010.2330871
CTCGTTT38050.010.2324451
Produced by FastQC (version 0.11.3)