FastQCFastQC Report
Wed 26 Apr 2017
liver_B322_mmu_LIsE.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_B322_mmu_LIsE.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences37257704
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC54650.022.15106844
CTCGTAT54800.021.66856842
AATCTCG67300.018.22394639
GATCGGA78150.018.1814561
ATCTCGT67950.017.80265840
TCGTATG77850.015.70816743
ACGTCTG86550.015.65621115
GCACACG99800.013.956711
ATCGGAA102700.013.8763342
ACACGTC105950.013.25139813
CGTCTGA103550.012.995616
TCTCGTA96550.012.63229441
CGGAAGA118400.012.4614484
ACCAATC108450.012.00831436
GACCAAT110400.011.74683335
CACACGT122900.011.43531312
CCAATCT115150.011.39237
CACGTCT128450.010.99638314
ACTGACC132100.010.94366932
TTACGGC21150.010.15309640
Produced by FastQC (version 0.11.3)