FastQCFastQC Report
Wed 26 Apr 2017
liver_B323_mmu_BxXL.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_B323_mmu_BxXL.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences41550594
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT48350.011.31209851
ACGTATA13700.011.10880518
GGACATA107600.011.09657321
GACATAT106700.011.08614822
TCGGTTA18000.010.9434562
AGCTTTA140100.010.8436654
CTCGTCT81300.010.6710951
CTCGTTT45400.010.4777781
CGTATAG13850.010.40535219
CTCGGTT46350.010.3534461
CTTAGAT93750.010.3492511
CTCAGCT347400.010.1942691
TCTTCGT107050.010.09648644
CTCCGAT84000.010.0786251
ACGTCGC13900.09.97841825
GCTTTAA153850.09.8579135
TAAACTC130600.09.78414259
CTCGCAT39900.09.7687551
CGGTCTA19000.09.5889444
TCCGATT43950.09.583772
Produced by FastQC (version 0.11.3)