FastQCFastQC Report
Wed 26 Apr 2017
liver_B324_mmu_bhK7.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_B324_mmu_bhK7.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences37525370
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC443050.11806679054730174TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG75250.032.59749639
CGTATGC77900.032.1477544
CGATGTA85500.030.24335134
CTCGTAT80600.030.05439642
GATCGGA100650.027.3128531
CACCGAT99450.026.44327531
ATCTCGT93950.026.04841840
TCGTATG96350.025.8151843
TCACCGA102650.025.76998730
GTCACCG103450.025.27622229
ACGTCTG110200.024.32431815
ACCGATG112500.023.74784532
TCTCGTA108250.022.87733741
ACACGTC122800.022.081513
GCACACG125050.021.9590211
CGTCTGA124200.021.7233616
GATGTAT121050.021.5065435
ATCGGAA128000.021.4439872
CCGATGT124500.021.00599133
CGGAAGA142550.019.3437544
Produced by FastQC (version 0.11.3)