FastQCFastQC Report
Wed 26 Apr 2017
liver_B325_mmu_FZdj.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_B325_mmu_FZdj.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences27708934
Sequences flagged as poor quality0
Sequence length15-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT346010.12487308245059157No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT320880.11580380537194249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA78850.018.8123912
CACGATC84300.018.7991221
CGATCAC85900.017.4160123
ATTAACG74800.015.70965220
ATTGGAC132400.014.2309086
ATACGTC70250.014.130321531
GGCATAC96550.013.92390230
CATACGT78450.013.91443730
TAACGAA85850.013.89445422
TAATGGG101300.013.88895119
CATACTA98150.013.75173832
TTAACGA86400.013.73839321
CGGTCTA17550.013.71308744
TAATGTA110850.013.6484334
GAATGCA133400.013.58598532
ACTATTA107200.013.5839535
TACGTCA73850.013.441421532
TACTATT113250.013.17912234
CCACGAT54100.013.0062131
GAATTGG157300.012.976394
Produced by FastQC (version 0.11.3)