FastQCFastQC Report
Wed 26 Apr 2017
liver_C218_mmu_V_DT.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_C218_mmu_V_DT.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences38023020
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT46100.023.86641342
CGTATGC52650.021.97432744
GATCTCG58200.018.93374839
GATCGGA63600.018.6975691
ATCTCGT63200.017.61907640
TCGTATG70700.016.18762843
ACGTCTG74800.015.572268515
GCACACG85450.014.1145311
ATCGGAA87700.013.5835382
TCTCGTA84900.013.452929541
AGTGATC89200.012.88853736
CGTCTGA91050.012.75924216
ACACGTC97550.012.18335813
CGGAAGA105700.011.5886144
CGATCAC53650.011.2588933
ACGATCA49050.011.2423832
CACACGT114550.010.41188812
CACGATC61050.010.4115421
CACGTCT119950.010.04113714
GCGACGT12750.09.69901722
Produced by FastQC (version 0.11.3)