FastQCFastQC Report
Wed 26 Apr 2017
liver_C219_mmu_bq8m.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_C219_mmu_bq8m.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences49643372
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG52150.026.89942439
CTCGTAT56850.024.63285842
CGCCAAT65800.021.9384233
CGTATGC71950.021.02507644
GTCACGC69350.020.86812829
TCACGCC74150.019.61961430
ATCTCGT74250.019.43208140
GATCGGA74600.019.1754231
TCGTATG86700.017.42819443
ACGTCTG83250.017.25397915
AGTCACG84750.017.2263828
ACGCCAA86700.016.79797432
CACGCCA94850.015.71915431
ATATCTC96300.015.13271638
GCACACG101000.014.48791311
ATCGGAA100300.014.3251162
ACGATCA74100.014.0244912
CACGATC84700.012.9646851
CCAATAT113750.012.90168735
AATATCT117050.012.77715437
Produced by FastQC (version 0.11.3)