FastQCFastQC Report
Wed 26 Apr 2017
liver_C220_mmu_Jqpf.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_C220_mmu_Jqpf.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences40144564
Sequences flagged as poor quality0
Sequence length15-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC82200.028.01245344
CTCGTAT81300.027.88513242
GATCGGA106750.024.943721
TCGTATG100500.023.16991843
ACGTCTG124250.021.45580315
CGTCTGA135800.019.5808516
GCACACG134500.019.56210111
ACACGTC137300.019.2374513
GACCAAT135500.018.84484335
TCTCGTA127550.018.38020541
ATCTCGT126200.018.23176840
ACCAATC138200.018.00949936
CGGAAGA154050.017.6177714
CCAATCT141900.017.52982537
ATCGGAA152350.017.4100512
CACACGT153100.017.24709312
CACGTCT162500.016.47893714
TCGGAAG174100.015.6855763
AATCTCG160700.014.79484239
GAACTCC195550.013.95381221
Produced by FastQC (version 0.11.3)