FastQCFastQC Report
Wed 26 Apr 2017
liver_C221_mmu_nxwG.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_C221_mmu_nxwG.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences34132428
Sequences flagged as poor quality0
Sequence length15-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC498930.14617477549502192TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC85250.032.46587444
CTCGTAT99300.027.38600542
GATCGGA125700.025.2331451
TCGTATG114150.024.2591743
ACGTCTG143650.021.41848415
ACACGTC148850.020.73900413
ATCTCGT133050.020.6145940
CGTCTGA149050.020.47642916
GCACACG154850.020.14619311
TCTCGTA140050.019.8490341
ACCAATC147250.019.7065636
GACCAAT147050.019.70117435
CGGAAGA164800.019.220024
CCAATCT150300.019.1889637
CACACGT164350.018.73565912
CACGTCT166950.018.5423114
ATCGGAA173750.018.303542
TCGGAAG184750.017.3391723
AATCTCG164900.017.07629839
ACTGACC188650.016.4228932
Produced by FastQC (version 0.11.3)