FastQCFastQC Report
Wed 26 Apr 2017
liver_C222_mmu_hMf8.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_C222_mmu_hMf8.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences41307509
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT65300.025.71642942
CGTATGC68700.025.0409344
GATCTCG73700.022.6472839
GATCGGA80750.022.158941
ATCTCGT80800.021.23250840
TCGTATG84550.020.3315443
ACGTCTG94050.018.61931215
TCTCGTA100050.017.06585141
GCACACG104850.016.9294511
ACACGTC110450.015.91121613
ATCGGAA113000.015.8349132
CGTCTGA110200.015.78716716
AGTGATC113050.014.96269236
CGGAAGA128400.014.3953794
CACACGT130350.013.66851812
ACGATCA68250.013.340362
CACGTCT134250.013.2074114
CACGATC75450.012.8493871
CGATCAC72800.012.7961073
TCGGAAG164650.011.2003943
Produced by FastQC (version 0.11.3)