FastQCFastQC Report
Wed 26 Apr 2017
liver_D252_mmu_8nhN.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_D252_mmu_8nhN.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences31931971
Sequences flagged as poor quality0
Sequence length15-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT35700.017.79128842
CGTATGC40500.016.49470344
GATCGGA45500.015.2878661
ATCTCGT43250.015.04974340
ACGTCTG50900.013.57070115
TCGTATG61900.011.15958143
GCACACG66550.010.59634611
CATCTCG63050.010.4826439
ATCGGAA68000.010.1985592
CGGCGAA26800.010.13397427
TTACGGC18650.09.77224540
TCATACG38350.09.65414510
GCGAAAC31300.09.31068729
ATTACGG20650.09.27391539
CGGAAGA80750.09.16181854
CTCGTAA35350.09.06058143
ACACGTC79900.08.98461613
TCTCGTA73800.08.90303541
CGTCTGA79500.08.75101516
TCGGCGA31450.08.6910226
Produced by FastQC (version 0.11.3)