FastQCFastQC Report
Wed 26 Apr 2017
liver_D253_mmu_Hvzy.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_D253_mmu_Hvzy.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences37189813
Sequences flagged as poor quality0
Sequence length15-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC506360.13615556496613737TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC90800.031.79234344
CTCGTAT92500.030.41329442
ATCTCGT100350.028.13772640
GATCTCG100350.028.01723939
GATCGGA110500.026.0146731
TCGTATG113650.025.5041543
ACGTCTG119800.023.79511615
AGTGATC123550.022.7332536
ACACGTC131900.021.55643713
TCTCGTA134300.021.3261341
CGTCTGA134100.021.19640716
ATCGGAA137200.020.8769022
GCACACG137850.020.65122211
CGGAAGA148850.019.324354
CACGTCT150800.019.07408514
CACACGT151650.018.79584112
TCGGAAG180100.016.2248123
TCTGAAC183250.015.90223918
GTCTGAA187300.015.65911117
GTGATCT183200.015.46091737
Produced by FastQC (version 0.11.3)