FastQCFastQC Report
Wed 26 Apr 2017
liver_D254_mmu_T7ft.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_D254_mmu_T7ft.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences26199056
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG27650.026.80358539
CTCGTAT32150.022.13889342
CGCCAAT33100.021.6382233
GTCACGC34350.021.12264629
CGTATGC38200.019.72064644
GATCGGA37000.019.355381
ATCTCGT38550.019.16435440
TCACGCC38500.018.7358530
AGTCACG39750.018.3944328
ACGCCAA44100.016.4141632
ACGTCTG46150.015.56873715
ATATCTC51350.014.99266538
CACGCCA49850.014.64909131
TCGTATG52650.014.52495243
ACACGTC54100.013.7253913
ATCGGAA53100.013.2555972
TCTCGTA58250.013.19431141
GCACACG56150.012.57059811
CGTCTGA56650.012.37190216
CGGAAGA61950.011.6914484
Produced by FastQC (version 0.11.3)