FastQCFastQC Report
Wed 26 Apr 2017
liver_D255_mmu_X01L.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_D255_mmu_X01L.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences37277123
Sequences flagged as poor quality0
Sequence length15-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG42100.028.16525839
CTCGTAT50050.023.30183842
CGCCAAT52250.022.69372233
GTCACGC54000.021.8650529
GATCGGA54850.021.57211
CGTATGC58300.021.1708144
ATCTCGT57600.020.38682740
AGTCACG61350.019.5087528
TCACGCC60400.019.48470930
ACGTCTG66050.017.93555515
ACGCCAA68450.017.4286532
ATATCTC72300.016.73430338
TCGTATG77950.015.87118743
CACGCCA77550.015.60922931
GCACACG83100.014.48851611
ATCGGAA82950.014.2643722
TCTCGTA86400.013.89744941
AATATCT92300.013.51534837
CCAATAT91800.013.07896935
ACACGTC92400.012.95667213
Produced by FastQC (version 0.11.3)