FastQCFastQC Report
Wed 26 Apr 2017
liver_D256_mmu_Jauq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_D256_mmu_Jauq.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences44655299
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC551060.12340304786672684TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT95100.031.4677642
CGTATGC97700.031.36553244
GATCGGA110400.029.2344571
ATCTCGT110900.027.33055540
TCGTATG117650.026.08605643
ACGTCTG122900.025.89272715
TCTCGTA132950.022.91066441
GCACACG141450.022.56282411
CATCTCG137050.022.4205739
ACACGTC142450.022.37230513
ATCGGAA145650.022.1592032
CGTCTGA143950.021.97322816
CGGAAGA155450.020.8303814
CACACGT164350.019.53033312
CACGTCT164200.019.45294214
GATCATC178850.017.7610436
TCGGAAG197800.016.4990023
AGAGCAC229800.014.6791958
GAACTCC226900.014.56906721
AGATCAT227600.014.29600535
Produced by FastQC (version 0.11.3)