FastQCFastQC Report
Wed 26 Apr 2017
liver_E10_mmu_08SL.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_E10_mmu_08SL.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences50718143
Sequences flagged as poor quality0
Sequence length15-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT66750.011.7995561
CGCGTGT24650.011.3378561
CTAGCCT140500.011.0007041
CTTAGAT104300.010.9821391
TAAGGCG108500.010.9634429
CTCGTTT54400.010.6641341
GCGCATT96200.010.53524944
CGATAAC24150.010.2575271
GACGTCG23650.010.25277824
GCGACGT18850.010.14654322
TAGCCTC139550.010.1349752
CTCGGTT64150.09.868441
GACATAT127000.09.84035722
TCGGTTA26300.09.6605442
AGCTTTA165800.09.6145454
GTTCCGC100700.09.60614627
TACCGAC39800.09.59083641
TTTAGGC130950.09.5879392
AGGCGTC125450.09.457890511
ACGTCGC20450.09.45742225
Produced by FastQC (version 0.11.3)