FastQCFastQC Report
Wed 26 Apr 2017
liver_E11_mmu_Ix4y.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_E11_mmu_Ix4y.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences24848988
Sequences flagged as poor quality0
Sequence length15-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC45450.019.37625544
CTCGTAT44450.018.47692742
GATCTCG49350.016.74794439
TCGTATG53750.016.2271843
GATCGGA54350.015.2189851
GCGACGT26050.015.02522422
GACGTCG28250.014.77426824
ACGTCGC26250.014.659389525
AGTCACG57550.014.39880828
GTCACGA57300.014.21249229
CGGTCTA25600.014.19252444
ACGATCA59300.013.79644732
TCGCTAT28750.013.52743328
GTCGCTA29950.013.17634927
AAAAGCG31500.012.66007218
AAGCGAC31750.012.57316320
CGACGTC31250.012.54680423
CGATCAG65650.012.49953233
ATCTCGT67300.012.46109440
CGCGCTA11500.012.3119323
Produced by FastQC (version 0.11.3)