FastQCFastQC Report
Wed 26 Apr 2017
liver_E7_mmu_8G94.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_E7_mmu_8G94.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences51349153
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT62650.012.3803611
TAAGGCG101100.010.9610439
CTTAGAT106300.010.9050421
CATACGT45550.010.4822430
ATACGTC32450.09.91581731
GACATAT127000.09.86040922
CTCGTTT54000.09.8503861
CTAGCCT144200.09.8171291
AGCTTTA166950.09.6471254
TTTATCG56900.09.64245417
CTCAGCT411850.09.611941
AGGCGTC113450.09.60646211
CGCGTGT24000.09.5364291
ATTAACG35450.09.486526520
GGACATA135850.09.39946821
CTCGGAT92250.09.3957231
TCGGTTA22700.09.3357472
CTCGGTT58350.09.2613131
CTCGAAC49100.09.2364611
TTTAGGC127000.09.1777022
Produced by FastQC (version 0.11.3)