FastQCFastQC Report
Wed 26 Apr 2017
liver_E8_mmu_ZnFv.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_E8_mmu_ZnFv.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences50283211
Sequences flagged as poor quality0
Sequence length15-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA539660.1073240927274911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT81350.032.50874342
CGTATGC89100.030.8131944
TCGTATG107150.025.55887443
GATCGGA105550.025.2300241
TCGCTAT136900.021.55986828
AAGCGAC139000.021.07497420
CATCTCG128950.020.97930539
CGCTATG142150.020.93683429
ACACGTC126300.020.76640113
AAAAGCG145000.020.03939218
GAACGCT153950.019.92957135
TTGGCCG159700.019.62521441
CGTCTGA133750.019.50701316
GTCGCTA157850.018.87376627
ACGTCTG142950.018.47781815
GCACACG145300.018.27737811
GATCATC151100.018.26752336
CACACGT144800.018.19923212
GCGACGT160550.018.1469622
ACGTCGC162600.018.0835325
Produced by FastQC (version 0.11.3)