FastQCFastQC Report
Wed 26 Apr 2017
liver_E9_mmu_kn2B.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameliver_E9_mmu_kn2B.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences54267958
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT65050.012.2744911
CTTAGAT114300.010.7442781
TTTATCG47150.010.34717717
CTCGTTT60950.010.2463311
TACCGAC35650.09.93652241
ACGATTG38600.09.4468663
CTCGTCT94650.09.3658151
TAAGGCG86200.09.3333129
CGATTGG54950.09.2292914
CGCGTGT24600.09.2005641
CGTAACG15250.09.13164730
CGATAAC25050.09.1189451
CTCAGAT178700.08.9597231
GCGACGT19400.08.95467322
CTCGGTT64950.08.9377141
CTCAGCT402850.08.8904661
TCGGTTT72900.08.8829412
CGGATAT52950.08.7864381
GCGCATT78000.08.77985744
AGCTTTA165000.08.7509644
Produced by FastQC (version 0.11.3)