FastQCFastQC Report
Thu 8 Dec 2016
15_240214_1HS_AGTCAA_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename15_240214_1HS_AGTCAA_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50456642
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG92350.024.6797333
CGTATGC113900.020.2356511
ATGCCGT122400.019.6681844
GCCGTCT137650.017.5037026
CCGTCTT141200.017.2070247
TGCCGTC144400.016.749555
GTATGCC143550.015.9869392
CGTCTTC195350.012.978588
CTCGAAT68050.012.7923161
GCTTGAA213150.011.85304816
CTCGTTT85800.011.24984551
TCGAATG61750.010.7486362
CGAATGT60100.010.6319213
TGCTTGA241200.010.539906515
CTCCGAT74100.010.5304751
CTTGAAA246450.010.3975917
CGACGAT29250.010.0232261
CTCGGTT66550.09.827431
CTCGGAT93100.09.6410291
CGGAAAT58800.09.2433581
Produced by FastQC (version 0.11.3)