FastQCFastQC Report
Tue 31 Oct 2017
Pool2_Index9_GATCAG_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenamePool2_Index9_GATCAG_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15199626
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA5241163.4482164232198875RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC2351551.5471104354804521RNA PCR Primer, Index 9 (100% over 50bp)
TACCCTGTAGAACCGAATGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1642811.0808226465572244RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGCGTGGAATTCTCGGGTGCCAAGGAACTCC1196530.7872101589868067RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGCTGGAATTCTCGGGTGCCAAGGAACTCCA914540.6016858572704354RNA PCR Primer, Index 1 (100% over 28bp)
GGCAATACCGGGGTTGTAGGACTATGGAATTCTCGGGTGCCAAGGAACTC908330.5976002304267224RNA PCR Primer, Index 1 (100% over 26bp)
GCAATACCGGGGTTGTAGGACTACATGGAATTCTCGGGTGCCAAGGAACT787550.5181377489156641RNA PCR Primer, Index 1 (100% over 25bp)
GTTACGGCAATACCGGGGTTGTAGGACTATGGAATTCTCGGGTGCCAAGG499390.32855413679257633Illumina Small RNA Adapter 2 (100% over 21bp)
TTACGGCAATACCGGGGTTGTAGGACTATGGAATTCTCGGGTGCCAAGGA487490.32072499678610517RNA PCR Primer, Index 1 (100% over 22bp)
ACCCTGTAGAACCGAATTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA398710.2623156648722804RNA PCR Primer, Index 1 (100% over 28bp)
GTTACGGCAATACCGGGGTTGTAGGACTACTGGAATTCTCGGGTGCCAAG351490.23124911099786272No Hit
GGCAATACCGGGGTTGTAGGACTACTGGAATTCTCGGGTGCCAAGGAACT303600.19974175680375295RNA PCR Primer, Index 1 (100% over 25bp)
TTACGGCAATACCGGGGTTGTAGGACTACTGGAATTCTCGGGTGCCAAGG301590.1984193558446767Illumina Small RNA Adapter 2 (100% over 21bp)
CAATACCGGGGTTGTAGGACTACATGGAATTCTCGGGTGCCAAGGAACTC298770.1965640470364205RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGGTTGTAGGACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGT283870.18676117425520866RNA PCR Primer, Index 1 (100% over 30bp)
GAAGAGTCGTTCGAGACCAGGACGTTGATAGGCTGGGTGTGGAATGGAAT245430.1614710783015319No Hit
TAAGTGCTAACTGTTGGGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA212860.14004291947709765RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGGTTGTAGGACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC211400.13908236952672387RNA PCR Primer, Index 1 (100% over 31bp)
GCAATACCGGGGTTGTAGGACTATGGAATTCTCGGGTGCCAAGGAACTCC203500.13388487321990686RNA PCR Primer, Index 1 (100% over 27bp)
TGTTACGGCAATACCGGGGTTGTAGGACTATGGAATTCTCGGGTGCCAAG200550.13194403599141188No Hit
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCC193560.1273452386262662RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGGTTGTAGGACTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA184120.12113455949508231RNA PCR Primer, Index 1 (100% over 32bp)
TTACGGCAATACCGGGGTTGTAGGACTACATGGAATTCTCGGGTGCCAAG183040.12042401569617568No Hit
CCCGTGTGAAAGTAGGACATCGTCAGGCTTGGAATTCTCGGGTGCCAAGG180970.11906214008160464Illumina Small RNA Adapter 2 (100% over 21bp)
GTAAACGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC180650.1188516085856323RNA PCR Primer, Index 9 (100% over 37bp)
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA178810.11764105248379136RNA PCR Primer, Index 1 (100% over 28bp)
GTTACGGCAATACCGGGGTTGTAGGACTACATGGAATTCTCGGGTGCCAA170660.11227907844574597No Hit
AGTAAACGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT168370.11077246242769394RNA PCR Primer, Index 9 (100% over 36bp)
ACTTGAAGGGTTTGATCCTGGCTCAGAACGAACGTTGGCGGCGTGGATTG163790.1077592303915899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTG1068050.042.704841
CTTGTCC30200.042.6128968
ATTTGTT605400.041.78103317
ACTTGTC35700.041.7785727
TTTGTTG605650.041.6588618
TATACAC31050.041.16431820
AACATTC19350.040.8504871
TTGTCTC5200.040.6125269
TTGTCCC31600.040.585759
TCGCATA2450.040.4053238
CTCGTAT267850.040.3838642
AGTGCTA59550.040.19583
TCTCGTA269850.040.11676841
ACATTCA19700.040.097652
ATCTCGT270750.039.9020340
CGTATGC272550.039.81660544
TACACGT31450.039.73089622
ACGTCTT8100.039.65844329
ATACACG31650.039.6193721
CGCAGCC24000.039.59721811
Produced by FastQC (version 0.11.3)