FastQCFastQC Report
Tue 31 Oct 2017
Pool2_Index9_GATCAG_L006_R1_001_prinseq_good_KXW5.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenamePool2_Index9_GATCAG_L006_R1_001_prinseq_good_KXW5.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13104468
Sequences flagged as poor quality0
Sequence length15-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGAACCGAATTTGT5437124.149058168557472No Hit
TACCCTGTAGAACCGAATGTGT1696341.2944745257876933No Hit
TACCCTGTAGAACCGAATTTGCG1239430.9458071857629017No Hit
TACCCTGTAGAACCGAATTTGC949250.7243712602449791No Hit
GGCAATACCGGGGTTGTAGGACTA915900.6989219249495668No Hit
GCAATACCGGGGTTGTAGGACTACA808690.6171101337345399No Hit
GTTACGGCAATACCGGGGTTGTAGGACTA516170.3938885577041357No Hit
TTACGGCAATACCGGGGTTGTAGGACTA504090.38467032770807635No Hit
ACCCTGTAGAACCGAATTTGCG413190.3153046731847489No Hit
GTTACGGCAATACCGGGGTTGTAGGACTAC361580.2759211591039026No Hit
TTACGGCAATACCGGGGTTGTAGGACTAC311600.2377814955937166No Hit
GAAGAGTCGTTCGAGACCAGGACGTTGATAGGCTGGGTG310600.23701839708410904No Hit
GGCAATACCGGGGTTGTAGGACTAC305050.23278320035578703No Hit
CAATACCGGGGTTGTAGGACTACA300400.22923479228611188No Hit
ACCGGGGTTGTAGGACTACA297580.22708285448901855No Hit
GCAATACCGGGGTTGTAGGACTA228920.1746885108193633No Hit
ACCGGGGTTGTAGGACTAC223300.1703998971953688No Hit
TAAGTGCTAACTGTTGGGGTAG217050.16563053151032153No Hit
TGTTACGGCAATACCGGGGTTGTAGGACTA206220.15736617465127162No Hit
ACCCTGTAGAACCGAATTTGTGT200090.15268838078737726No Hit
ACCGGGGTTGTAGGACTA195480.1491704966580864No Hit
TTACGGCAATACCGGGGTTGTAGGACTACA188210.1436227704932394No Hit
CCCGTGTGAAAGTAGGACATCGTCAGGCT186790.14253917060959667No Hit
ACCCTGTAGAACCGAATTTGTG184750.14098244964999723No Hit
GTTACGGCAATACCGGGGTTGTAGGACTACA175580.1339848363168959No Hit
ACTTGAAGGGTTTGATCCTGGCTCAGAACGAACGTTGGCGGCGTGGATTG161940.12357617264584872No Hit
TATTGCACTTGTCCCGGCCTGT156690.11956990547040902No Hit
ACCCTGTAGAACCGAATTTGT156260.11924177311127776No Hit
GCAATACCGGGGTTGTAGGACTAC155020.1182955309593644No Hit
GCGGGTGTAGCTCAGTTGGTTAGAGTGCC152960.11672354802957281No Hit
TACGGCAATACCGGGGTTGTAGGACTA151710.11576967489256337No Hit
TACCCTGTAGATCCGGATTTGT144070.1099396022791616No Hit
ACCGGGGTTGTAGGACT141830.10823026161764064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGATT28650.0588.6332443
AGCCCCT5100.0550.993341
CGTGGAT35600.0528.8359442
CTACTAT201.7991758E-4494.5838644
TAGCCCC8100.0429.5359540
AGCCCTC2150.0428.1575641
GGCGTGG44450.0425.0666540
CGGCGTG45650.0414.9256339
CTCGTAT3600.0381.32542
GCCCCTG6850.0372.1795742
ATAGCCC16600.0365.831439
GCGTGGA53150.0351.8621541
GCGGCGT44450.0351.8527838
AGCGTAT4700.0348.6579339
CCCCACG702.0736479E-10345.162840
GCTCCAT1900.0336.40743
TAGCCCT7700.0326.3357240
AATAGCC17150.0319.2955638
TGGATTG51300.0309.4764444
GATGGAA13400.0298.964944
Produced by FastQC (version 0.11.3)