MYCN

Name	Synonyms	Full Name	RefSeq ID	Description (.pdf)	IGV-img (human_genome)	Sashimi-img (human_genome)	UCSC-img (human_genome)	IGV-img (bat_genome)
MYCN	NMYC, ODED, MODED, N-myc, bHLHe37,bHLHe37, MYCNOT	v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog	NM_005378	pdf

Download all snapshots for IGV, UCSC and Sashimi (zip archive)

Description

This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

The transcriptional regulator MYCN has to dimerize, preferentially with the Max protein, to bind to DNA by a basic helix-loop-helix motif [Ma et al., Oncogene, 1993, 8:1093-1098]. N-myc has been shown to both up- and down-regulate many target genes, in addition, this factor modulates the expression of several classes of non-coding RNAs, in particular microRNAs [Buechner and Einvik, Mol Cancer Res, 2012, 10:1243-1253]. N-myc mRNA was strongly down-regulated (20X) in human cells infected with EBOV, whereas in MARV-infected cells, only slight down-regulation was observed (3X). This gene is not part of the bat transcriptome assembly. Due to the very strong down-regulation of n-myc, it seems to be very important for an EBOV infection to deplete this factor from infected cells.

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Maximum read counts and DESeq normalized read counts for human and bat cell lines

Source	Species	Mapping on	Mock_3h	Mock_7h	Mock_23h	EBOV_3h	EBOV_7h	EBOV_23h	MARV_3h	MARV_7h	MARV_23h
Read_Max	H. sapiens	Genome	238	275	373	195	340	15	218	322	102
Read_Max	R. aegyptiacus	Transcriptome	1	1	1	1	1	1	2	1	2
Read_Max	R. aegyptiacus	Genome	1	0	1	1	0	0	2	0	0
DESeq	H. sapiens	Genome	2852.55	3707.22	4918.46	2654.23	3531.65	69.43	2369.45	3366.27	1338.44
DESeq	R. aegyptiacus	Transcriptome	1.0	1.0	2.22	1.0	1.0	1.0	3.22	1.0	2.75
DESeq	R. aegyptiacus	Genome	2.58	0.0	1.12	0.99	0.0	0.0	2.25	0.0	0.0

Human Condition Comparisons

	Mock	EBOV	MARV	FC_{Mock_EBOV}	FC_{Mock_MARV}	FC_{EBOV_MARV}
3h	2852.55	2654.23	2369.45	-0.1	-0.27	-0.16
7h	3707.22	3531.65	3366.27	-0.07	-0.14	-0.07
23h	4918.46	69.43	1338.44	-6.15	-1.88	4.27

Human Time Point Comparisons

	3h	7h	23h	FC_{3h_7h}	FC_{3h_23h}	FC_{7h_23h}
Mock	2852.55	3707.22	4918.46	0.38	0.79	0.41
Ebov	2654.23	3531.65	69.43	0.41	-5.26	-5.67
Marv	2369.45	3366.27	1338.44	0.51	-0.82	-1.33

Bat Condition Comparisons

	Mock	EBOV	MARV	FC_{Mock_EBOV}	FC_{Mock_MARV}	FC_{EBOV_MARV}
3h	1.0	1.0	3.22	0.0	1.69	1.69
7h	1.0	1.0	1.0	0.0	0.0	0.0
23h	2.22	1.0	2.75	-1.15	0.31	1.46

Bat Time Point Comparisons

	3h	7h	23h	FC_{3h_7h}	FC_{3h_23h}	FC_{7h_23h}
Mock	1.0	1.0	2.22	0.0	1.15	1.15
Ebov	1.0	1.0	1.0	0.0	0.0	0.0
Marv	3.22	1.0	2.75	-1.69	-0.23	1.46

Differential transcriptional responses to Ebola and Marburg virus infection in cells from bats and humans

Supplemental Material