IDH1

NameSynonymsFull NameRefSeq IDDescription (.pdf)IGV-img (humangenome)Sashimi-img (humangenome)UCSC-img (humangenome)IGV-img (batgenome)
IDH1 isocitrate dehydrogenase 1 (NADP+), soluble pdf

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Description

Isocitrate dehydrogenases, here IDH1 was analyzed, catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

This gene was expressed in all probes. The most significant differential expression in human cells was observed for the 23 h infected Ebola probe, as it was 8x downregulated. For the samples (WT and MARV) at this timepoint no or only a slight downregulation was observed. In the bat cells the gene seems to be equally expressed.

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Maximum read counts and DESeq normalized read counts for human and bat cell lines

Source Species Mapping on Mock3h Mock7h Mock23h EBOV3h EBOV7h EBOV23h MARV3h MARV7h MARV23h
Read_Max
humanH. sapiensGenome2149 2582 3044 2166 3442 431 2933 3144 1729
Read_Max
batR. aegyptiacusTranscriptome 367 288 292 352 304 340 302 353 384
Read_Max
bat_genomeR. aegyptiacusGenome 407 376 250 281 302 221 263 506 523
DESeq
humanH. sapiensGenome 20620.45 23770.16 29707.31 22852.3 23486.33 3626.37 22171.47 21625.03 17695.4
DESeq
batR. aegyptiacusTranscriptome 2009.78 1938.01 2459.95 2296.58 2433.19 2596.88 2256.96 2314.38 2774.7
DESeq
bat_genomeR. aegyptiacusGenome 2956.3 3441.73 3391.96 2983.19 3732.35 4096.38 3438.82 3451.69 4264.69

Human Condition Comparisons

human Mock EBOV MARV FCMock_EBOV FCMock_MARV FCEBOV_MARV
3h 20620.45 22852.3 22171.47 0.15 0.1 -0.04
7h 23770.16 23486.33 21625.03 -0.02 -0.14 -0.12
23h 29707.31 3626.37 17695.4 -3.03 -0.75 2.29

Human Time Point Comparisons

human 3h 7h 23h FC3h_7h FC3h_23h FC7h_23h
Mock 20620.45 23770.16 29707.31 0.21 0.53 0.32
Ebov 22852.3 23486.33 3626.37 0.04 -2.66 -2.7
Marv 22171.47 21625.03 17695.4 -0.04 -0.33 -0.29

Bat Condition Comparisons

bat Mock EBOV MARV FCMock_EBOV FCMock_MARV FCEBOV_MARV
3h 2009.78 2296.58 2256.96 0.19 0.17 -0.03
7h 1938.01 2433.19 2314.38 0.33 0.26 -0.07
23h 2459.95 2596.88 2774.7 0.08 0.17 0.1

Bat Time Point Comparisons

bat 3h 7h 23h FC3h_7h FC3h_23h FC7h_23h
Mock 2009.78 1938.01 2459.95 -0.05 0.29 0.34
Ebov 2296.58 2433.19 2596.88 0.08 0.18 0.09
Marv 2256.96 2314.38 2774.7 0.04 0.3 0.26