GCLM

NameSynonymsFull NameRefSeq IDDescription (.pdf)IGV-img (humangenome)Sashimi-img (humangenome)UCSC-img (humangenome)IGV-img (batgenome)
GCLM A8K334, D3DT45, GLCLR, GSH0_HUMAN, NM_002061, NP_002052, P48507, Q6FHC1, Q9NPX9, Q9NU74 glutamate-cysteine ligase, modifier subunit NM_002061 pdf

Download all snapshots for IGV, UCSC and Sashimi (zip archive)

Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase,is the first rate limiting enzyme of glutathione synthesis. The enzymeconsists of two subunits, a heavy catalytic subunit and a light regulatorysubunit. Gamma glutamylcysteine synthetase deficiency has been implicated insome forms of hemolytic anemia.

This gene is alternatively spliced and shows different isoforms in human cellswildtype, which are subsequently limited during the Ebola infection by lossof one alternative splicing event. Regarding the expression, a high levelcan be observed as well as diverse regulations. The human cells and bat cells sampleshave in common wildtype downregulation, Ebola downregulation and Marburgupregulation. Between the human cells genome and bat cells transcriptome assembly themain difference is the drastic downregulation in human cells samples.

Show legend

Maximum read counts and DESeq normalized read counts for human and bat cell lines

Source Species Mapping on Mock3h Mock7h Mock23h EBOV3h EBOV7h EBOV23h MARV3h MARV7h MARV23h
Read_Max
humanH. sapiensGenome498 401 362 405 653 221 604 985 1020
Read_Max
batR. aegyptiacusTranscriptome 479 480 356 383 396 344 481 480 711
Read_Max
bat_genomeR. aegyptiacusGenome 553 415 315 474 340 205 429 591 819
DESeq
humanH. sapiensGenome 5798.48 5102.4 4316.81 5297.19 6243.65 2988.14 6725.65 9948.12 12605.52
DESeq
batR. aegyptiacusTranscriptome 2007.26 1928.03 1618.31 1938.55 1944.78 1353.47 2340.39 1837.66 2292.74
DESeq
bat_genomeR. aegyptiacusGenome 3972.1 3713.71 2857.51 3791.94 3739.05 2680.01 4656.69 3521.47 4626.0

Human Condition Comparisons

human Mock EBOV MARV FCMock_EBOV FCMock_MARV FCEBOV_MARV
3h 5798.48 5297.19 6725.65 -0.13 0.21 0.34
7h 5102.4 6243.65 9948.12 0.29 0.96 0.67
23h 4316.81 2988.14 12605.52 -0.53 1.55 2.08

Human Time Point Comparisons

human 3h 7h 23h FC3h_7h FC3h_23h FC7h_23h
Mock 5798.48 5102.4 4316.81 -0.18 -0.43 -0.24
Ebov 5297.19 6243.65 2988.14 0.24 -0.83 -1.06
Marv 6725.65 9948.12 12605.52 0.56 0.91 0.34

Bat Condition Comparisons

bat Mock EBOV MARV FCMock_EBOV FCMock_MARV FCEBOV_MARV
3h 2007.26 1938.55 2340.39 -0.05 0.22 0.27
7h 1928.03 1944.78 1837.66 0.01 -0.07 -0.08
23h 1618.31 1353.47 2292.74 -0.26 0.5 0.76

Bat Time Point Comparisons

bat 3h 7h 23h FC3h_7h FC3h_23h FC7h_23h
Mock 2007.26 1928.03 1618.31 -0.06 -0.31 -0.25
Ebov 1938.55 1944.78 1353.47 0.0 -0.52 -0.52
Marv 2340.39 1837.66 2292.74 -0.35 -0.03 0.32