GCLM

Name	Synonyms	Full Name	RefSeq ID	Description (.pdf)	IGV-img (human_genome)	Sashimi-img (human_genome)	UCSC-img (human_genome)	IGV-img (bat_genome)
GCLM	A8K334, D3DT45, GLCLR, GSH0_HUMAN, NM_002061, NP_002052, P48507, Q6FHC1, Q9NPX9, Q9NU74	glutamate-cysteine ligase, modifier subunit	NM_002061	pdf

Download all snapshots for IGV, UCSC and Sashimi (zip archive)

Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase,is the first rate limiting enzyme of glutathione synthesis. The enzymeconsists of two subunits, a heavy catalytic subunit and a light regulatorysubunit. Gamma glutamylcysteine synthetase deficiency has been implicated insome forms of hemolytic anemia.

This gene is alternatively spliced and shows different isoforms in human cellswildtype, which are subsequently limited during the Ebola infection by lossof one alternative splicing event. Regarding the expression, a high levelcan be observed as well as diverse regulations. The human cells and bat cells sampleshave in common wildtype downregulation, Ebola downregulation and Marburgupregulation. Between the human cells genome and bat cells transcriptome assembly themain difference is the drastic downregulation in human cells samples.

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Maximum read counts and DESeq normalized read counts for human and bat cell lines

Source	Species	Mapping on	Mock_3h	Mock_7h	Mock_23h	EBOV_3h	EBOV_7h	EBOV_23h	MARV_3h	MARV_7h	MARV_23h
Read_Max	H. sapiens	Genome	498	401	362	405	653	221	604	985	1020
Read_Max	R. aegyptiacus	Transcriptome	479	480	356	383	396	344	481	480	711
Read_Max	R. aegyptiacus	Genome	553	415	315	474	340	205	429	591	819
DESeq	H. sapiens	Genome	5798.48	5102.4	4316.81	5297.19	6243.65	2988.14	6725.65	9948.12	12605.52
DESeq	R. aegyptiacus	Transcriptome	2007.26	1928.03	1618.31	1938.55	1944.78	1353.47	2340.39	1837.66	2292.74
DESeq	R. aegyptiacus	Genome	3972.1	3713.71	2857.51	3791.94	3739.05	2680.01	4656.69	3521.47	4626.0

Human Condition Comparisons

	Mock	EBOV	MARV	FC_{Mock_EBOV}	FC_{Mock_MARV}	FC_{EBOV_MARV}
3h	5798.48	5297.19	6725.65	-0.13	0.21	0.34
7h	5102.4	6243.65	9948.12	0.29	0.96	0.67
23h	4316.81	2988.14	12605.52	-0.53	1.55	2.08

Human Time Point Comparisons

	3h	7h	23h	FC_{3h_7h}	FC_{3h_23h}	FC_{7h_23h}
Mock	5798.48	5102.4	4316.81	-0.18	-0.43	-0.24
Ebov	5297.19	6243.65	2988.14	0.24	-0.83	-1.06
Marv	6725.65	9948.12	12605.52	0.56	0.91	0.34

Bat Condition Comparisons

	Mock	EBOV	MARV	FC_{Mock_EBOV}	FC_{Mock_MARV}	FC_{EBOV_MARV}
3h	2007.26	1938.55	2340.39	-0.05	0.22	0.27
7h	1928.03	1944.78	1837.66	0.01	-0.07	-0.08
23h	1618.31	1353.47	2292.74	-0.26	0.5	0.76

Bat Time Point Comparisons

	3h	7h	23h	FC_{3h_7h}	FC_{3h_23h}	FC_{7h_23h}
Mock	2007.26	1928.03	1618.31	-0.06	-0.31	-0.25
Ebov	1938.55	1944.78	1353.47	0.0	-0.52	-0.52
Marv	2340.39	1837.66	2292.74	-0.35	-0.03	0.32

Differential transcriptional responses to Ebola and Marburg virus infection in cells from bats and humans

Supplemental Material