MED12

Name	Synonyms	Full Name	RefSeq ID	Description (.pdf)	IGV-img (human_genome)	Sashimi-img (human_genome)	UCSC-img (human_genome)	IGV-img (bat_genome)
MED12	MED12, ARC240, CAGH45, FGS1, HOPA, MED12S, OHDOX, OKS, OPA1, TNRC11, TRAP230,CAGH45, KIAA0192	mediator complex subunit 12	NM_005120	pdf

Download all snapshots for IGV, UCSC and Sashimi (zip archive)

Description

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase.

Gene is upregulated during Ebola infection in human cells but not in the bat cells homologs. In bat cells, it is slightly upregulated during Marburg infection.

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Maximum read counts and DESeq normalized read counts for human and bat cell lines

Source	Species	Mapping on	Mock_3h	Mock_7h	Mock_23h	EBOV_3h	EBOV_7h	EBOV_23h	MARV_3h	MARV_7h	MARV_23h
Read_Max	H. sapiens	Genome	55	81	88	64	104	284	67	105	89
Read_Max	R. aegyptiacus	Transcriptome	43	30	21	21	26	37	28	40	46
Read_Max	R. aegyptiacus	Genome	111	107	63	72	73	60	68	130	137
DESeq	H. sapiens	Genome	1903.73	2612.89	2434.41	1916.21	2192.72	7537.29	1993.13	2484.58	2301.83
DESeq	R. aegyptiacus	Transcriptome	396.71	349.55	452.97	371.85	374.43	416.75	304.69	354.62	433.11
DESeq	R. aegyptiacus	Genome	2261.91	2348.34	2596.41	2206.04	2252.36	2417.62	1937.57	2271.0	2566.01

Human Condition Comparisons

	Mock	EBOV	MARV	FC_{Mock_EBOV}	FC_{Mock_MARV}	FC_{EBOV_MARV}
3h	1903.73	1916.21	1993.13	0.01	0.07	0.06
7h	2612.89	2192.72	2484.58	-0.25	-0.07	0.18
23h	2434.41	7537.29	2301.83	1.63	-0.08	-1.71

Human Time Point Comparisons

	3h	7h	23h	FC_{3h_7h}	FC_{3h_23h}	FC_{7h_23h}
Mock	1903.73	2612.89	2434.41	0.46	0.35	-0.1
Ebov	1916.21	2192.72	7537.29	0.19	1.98	1.78
Marv	1993.13	2484.58	2301.83	0.32	0.21	-0.11

Bat Condition Comparisons

	Mock	EBOV	MARV	FC_{Mock_EBOV}	FC_{Mock_MARV}	FC_{EBOV_MARV}
3h	396.71	371.85	304.69	-0.09	-0.38	-0.29
7h	349.55	374.43	354.62	0.1	0.02	-0.08
23h	452.97	416.75	433.11	-0.12	-0.06	0.06

Bat Time Point Comparisons

	3h	7h	23h	FC_{3h_7h}	FC_{3h_23h}	FC_{7h_23h}
Mock	396.71	349.55	452.97	-0.18	0.19	0.37
Ebov	371.85	374.43	416.75	0.01	0.16	0.15
Marv	304.69	354.62	433.11	0.22	0.51	0.29

Differential transcriptional responses to Ebola and Marburg virus infection in cells from bats and humans

Supplemental Material