MCM4

NameSynonymsFull NameRefSeq IDDescription (.pdf)IGV-img (humangenome)Sashimi-img (humangenome)UCSC-img (humangenome)IGV-img (batgenome)
MCM4 AK309812,CDC54, hCdc21, P1-Cdc21, MGC33310 minichromosome maintenance complex component 4 NM_005914 pdf

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Description

The protein encoded by the gene MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre\_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

For this gene we observed a downregulation after 23 h for all probes, which was most significant within the cells infected with the Marburg virus (3x). However this gene was also less expressed at this timepoint in the bat cells for all probes. Interestingly, there were splice variants, which were unique for the Marburg infected cells (exon 7-10) and Ebola infected cells (exon 6-9) after 7 h.

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Maximum read counts and DESeq normalized read counts for human and bat cell lines

Source Species Mapping on Mock3h Mock7h Mock23h EBOV3h EBOV7h EBOV23h MARV3h MARV7h MARV23h
Read_Max
humanH. sapiensGenome919 884 650 853 856 231 1022 1102 331
Read_Max
batR. aegyptiacusTranscriptome 1687 1500 690 1396 1245 946 1237 1592 1128
Read_Max
bat_genomeR. aegyptiacusGenome 1612 1301 519 1294 926 507 949 1738 1113
DESeq
humanH. sapiensGenome 11216.42 9984.54 9847.83 11055.36 9654.79 2936.82 11075.71 9570.02 5821.57
DESeq
batR. aegyptiacusTranscriptome 11270.64 11439.71 5815.51 9768.43 9890.76 6964.83 11826.99 9824.62 6871.84
DESeq
bat_genomeR. aegyptiacusGenome 13640.24 11740.78 6386.71 14475.52 11684.81 8494.3 14313.04 11511.2 7893.23

Human Condition Comparisons

human Mock EBOV MARV FCMock_EBOV FCMock_MARV FCEBOV_MARV
3h 11216.42 11055.36 11075.71 -0.02 -0.02 0.0
7h 9984.54 9654.79 9570.02 -0.05 -0.06 -0.01
23h 9847.83 2936.82 5821.57 -1.75 -0.76 0.99

Human Time Point Comparisons

human 3h 7h 23h FC3h_7h FC3h_23h FC7h_23h
Mock 11216.42 9984.54 9847.83 -0.17 -0.19 -0.02
Ebov 11055.36 9654.79 2936.82 -0.2 -1.91 -1.72
Marv 11075.71 9570.02 5821.57 -0.21 -0.93 -0.72

Bat Condition Comparisons

bat Mock EBOV MARV FCMock_EBOV FCMock_MARV FCEBOV_MARV
3h 11270.64 9768.43 11826.99 -0.21 0.07 0.28
7h 11439.71 9890.76 9824.62 -0.21 -0.22 -0.01
23h 5815.51 6964.83 6871.84 0.26 0.24 -0.02

Bat Time Point Comparisons

bat 3h 7h 23h FC3h_7h FC3h_23h FC7h_23h
Mock 11270.64 11439.71 5815.51 0.02 -0.95 -0.98
Ebov 9768.43 9890.76 6964.83 0.02 -0.49 -0.51
Marv 11826.99 9824.62 6871.84 -0.27 -0.78 -0.52