SMURF2

Name	Synonyms	Full Name	RefSeq ID	Description (.pdf)	IGV-img (human_genome)	Sashimi-img (human_genome)	UCSC-img (human_genome)	IGV-img (bat_genome)
SMURF2		SMAD specific E3 ubiquitin protein ligase 2	NM_022739	pdf

Download all snapshots for IGV, UCSC and Sashimi (zip archive)

Description

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Moderate to low expression in human cells samples and a more than 10-fold increase in expression in the bat cells homolog. However, there is no significant variance between the samples within one species.

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Maximum read counts and DESeq normalized read counts for human and bat cell lines

Source	Species	Mapping on	Mock_3h	Mock_7h	Mock_23h	EBOV_3h	EBOV_7h	EBOV_23h	MARV_3h	MARV_7h	MARV_23h
Read_Max	H. sapiens	Genome	87	60	51	53	66	75	83	76	72
Read_Max	R. aegyptiacus	Transcriptome	1285	1005	599	983	868	1280	978	1064	1060
Read_Max	R. aegyptiacus	Genome	1501	1047	524	1029	736	773	850	1303	1219
DESeq	H. sapiens	Genome	889.95	669.02	727.75	823.43	683.07	1077.42	845.75	626.89	1075.3
DESeq	R. aegyptiacus	Transcriptome	12231.76	10276.96	7723.56	10383.76	9991.51	12775.07	12543.38	9626.33	8787.45
DESeq	R. aegyptiacus	Genome	18366.88	15453.58	11358.62	15549.9	15431.67	18984.58	19199.14	14305.54	13998.73

Human Condition Comparisons

	Mock	EBOV	MARV	FC_{Mock_EBOV}	FC_{Mock_MARV}	FC_{EBOV_MARV}
3h	889.95	823.43	845.75	-0.11	-0.07	0.04
7h	669.02	683.07	626.89	0.03	-0.09	-0.12
23h	727.75	1077.42	1075.3	0.57	0.56	-0.0

Human Time Point Comparisons

	3h	7h	23h	FC_{3h_7h}	FC_{3h_23h}	FC_{7h_23h}
Mock	889.95	669.02	727.75	-0.41	-0.29	0.12
Ebov	823.43	683.07	1077.42	-0.27	0.39	0.66
Marv	845.75	626.89	1075.3	-0.43	0.35	0.78

Bat Condition Comparisons

	Mock	EBOV	MARV	FC_{Mock_EBOV}	FC_{Mock_MARV}	FC_{EBOV_MARV}
3h	12231.76	10383.76	12543.38	-0.24	0.04	0.27
7h	10276.96	9991.51	9626.33	-0.04	-0.09	-0.05
23h	7723.56	12775.07	8787.45	0.73	0.19	-0.54

Bat Time Point Comparisons

	3h	7h	23h	FC_{3h_7h}	FC_{3h_23h}	FC_{7h_23h}
Mock	12231.76	10276.96	7723.56	-0.25	-0.66	-0.41
Ebov	10383.76	9991.51	12775.07	-0.06	0.3	0.35
Marv	12543.38	9626.33	8787.45	-0.38	-0.51	-0.13

Differential transcriptional responses to Ebola and Marburg virus infection in cells from bats and humans

Supplemental Material