Differential transcriptional responses to Ebola and Marburg virus infection in cells from bats and humans

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PTPRF

Name	Synonyms	Full Name	RefSeq ID	Description (.pdf)	IGV-img (humangenome)	Sashimi-img (humangenome)	UCSC-img (humangenome)	IGV-img (batgenome)
PTPRF	LAR	protein tyrosine phosphatase, receptor type, F		pdf

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Description

The protein encoded by this gene is a member of the protein tyrosinephosphatase (PTP) family. PTPs are known to be signaling molecules thatregulate a variety of cellular processes including cell growth,differentiation, mitotic cycle, and oncogenic transformation. This PTPpossesses an extracellular region, a single transmembrane region, and twotandem intracytoplasmic catalytic domains, and thus represents areceptor-type PTP. The extracellular region contains three Ig-like domains,and nine non-Ig like domains similar to that of neural-cell adhesionmolecule. This PTP was shown to function in the regulation of epithelialcell-cell contacts at adherents junctions, as well as in the control ofbeta-catenin signaling. An increased expression level of this protein wasfound in the insulin-responsive tissue of obese, insulin-resistantindividuals, and may contribute to the pathogenesis of insulin resistance.Two alternatively spliced transcript variants of this gene, which encodedistinct proteins, have been reported.

The gene shows a really interesting differential expression and differentsplicing patterns for different states. PTPRF is upregulated after Ebolainfection in human cells, whereas the expression does not change in bat cells. In contrastMarburg infection leads to an expression decrease in human cells and an 2-foldup-regulation in bat cells 23 h after infection. An undescribed splice variantis observed only in human cells 23 h after infection with Ebola. In addition,the last exons show a diverse splicing pattern in different conditions.

Tyrosine phosphorylation during Ebola virus infection on VP40 can beperformed by c-ABl1 tyrosine kinase and is important for viral life cycle.Furthermore, reduced levels of protein tyrosine phosphatase CD45 protectmice from lethal effects of Ebola virus infection. Thus, events involved intyrosine phosphorylation of Ebola virus proteins seem to beimportant~\citegarcia2012,panchal2009.

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