MAP3K3

Name	Synonyms	Full Name	RefSeq ID	Description (.pdf)	IGV-img (human_genome)	Sashimi-img (human_genome)	UCSC-img (human_genome)	IGV-img (bat_genome)
MAP3K3	MEKK3,MAPKKK3	mitogen-activated protein kinase kinase kinase 3	NM_002401	pdf

Download all snapshots for IGV, UCSC and Sashimi (zip archive)

Description

This gene product is a 626-amino acid polypeptide that is 96.5\,\% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Constantly expressed in human cells and bat cells but at a relatively low level.

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Maximum read counts and DESeq normalized read counts for human and bat cell lines

Source	Species	Mapping on	Mock_3h	Mock_7h	Mock_23h	EBOV_3h	EBOV_7h	EBOV_23h	MARV_3h	MARV_7h	MARV_23h
Read_Max	H. sapiens	Genome	36	55	36	44	57	58	54	49	27
Read_Max	R. aegyptiacus	Transcriptome	225	227	116	153	137	189	177	203	200
Read_Max	R. aegyptiacus	Genome	276	191	102	225	121	115	166	279	232
DESeq	H. sapiens	Genome	633.21	741.43	610.59	671.72	687.13	1057.69	703.64	793.96	579.47
DESeq	R. aegyptiacus	Transcriptome	1855.19	2004.27	1304.38	1491.71	1482.66	1792.32	2033.37	1498.09	1574.6
DESeq	R. aegyptiacus	Genome	3014.74	2472.46	2188.04	3396.94	2663.1	3245.25	3543.4	2482.71	2700.43

Human Condition Comparisons

	Mock	EBOV	MARV	FC_{Mock_EBOV}	FC_{Mock_MARV}	FC_{EBOV_MARV}
3h	633.21	671.72	703.64	0.09	0.15	0.07
7h	741.43	687.13	793.96	-0.11	0.1	0.21
23h	610.59	1057.69	579.47	0.79	-0.08	-0.87

Human Time Point Comparisons

	3h	7h	23h	FC_{3h_7h}	FC_{3h_23h}	FC_{7h_23h}
Mock	633.21	741.43	610.59	0.23	-0.05	-0.28
Ebov	671.72	687.13	1057.69	0.03	0.65	0.62
Marv	703.64	793.96	579.47	0.17	-0.28	-0.45

Bat Condition Comparisons

	Mock	EBOV	MARV	FC_{Mock_EBOV}	FC_{Mock_MARV}	FC_{EBOV_MARV}
3h	1855.19	1491.71	2033.37	-0.31	0.13	0.45
7h	2004.27	1482.66	1498.09	-0.43	-0.42	0.01
23h	1304.38	1792.32	1574.6	0.46	0.27	-0.19

Bat Time Point Comparisons

	3h	7h	23h	FC_{3h_7h}	FC_{3h_23h}	FC_{7h_23h}
Mock	1855.19	2004.27	1304.38	0.11	-0.51	-0.62
Ebov	1491.71	1482.66	1792.32	-0.01	0.26	0.27
Marv	2033.37	1498.09	1574.6	-0.44	-0.37	0.07

Differential transcriptional responses to Ebola and Marburg virus infection in cells from bats and humans

Supplemental Material