MAP3K4

NameSynonymsFull NameRefSeq IDDescription (.pdf)IGV-img (humangenome)Sashimi-img (humangenome)UCSC-img (humangenome)IGV-img (batgenome)
MAP3K4 KIAA0213, MAPKKK4, MEKK4, MTK1,MTK1, KIAA0213 mitogen-activated protein kinase kinase kinase 4 pdf

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Description

The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described.

It is constantly expressed in human cells but no homolog was found in bat cells.

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Maximum read counts and DESeq normalized read counts for human and bat cell lines

Source Species Mapping on Mock3h Mock7h Mock23h EBOV3h EBOV7h EBOV23h MARV3h MARV7h MARV23h
Read_Max
humanH. sapiensGenome69 74 72 80 108 75 83 80 46
Read_Max
batR. aegyptiacusTranscriptome 154 146 120 157 136 215 114 192 142
Read_Max
bat_genomeR. aegyptiacusGenome 171 171 101 136 120 133 111 242 165
DESeq
humanH. sapiensGenome 1524.2 1592.0 1536.9 1783.89 1662.67 1560.37 1672.95 1488.59 1188.38
DESeq
batR. aegyptiacusTranscriptome 2814.63 2848.42 2887.49 3248.88 3287.36 4515.23 2635.18 3225.69 2947.89
DESeq
bat_genomeR. aegyptiacusGenome 3760.69 4084.26 3567.14 3810.7 4231.26 6046.66 3447.82 3997.24 3714.16

Human Condition Comparisons

human Mock EBOV MARV FCMock_EBOV FCMock_MARV FCEBOV_MARV
3h 1524.2 1783.89 1672.95 0.23 0.13 -0.09
7h 1592.0 1662.67 1488.59 0.06 -0.1 -0.16
23h 1536.9 1560.37 1188.38 0.02 -0.37 -0.39

Human Time Point Comparisons

human 3h 7h 23h FC3h_7h FC3h_23h FC7h_23h
Mock 1524.2 1592.0 1536.9 0.06 0.01 -0.05
Ebov 1783.89 1662.67 1560.37 -0.1 -0.19 -0.09
Marv 1672.95 1488.59 1188.38 -0.17 -0.49 -0.32

Bat Condition Comparisons

bat Mock EBOV MARV FCMock_EBOV FCMock_MARV FCEBOV_MARV
3h 2814.63 3248.88 2635.18 0.21 -0.1 -0.3
7h 2848.42 3287.36 3225.69 0.21 0.18 -0.03
23h 2887.49 4515.23 2947.89 0.64 0.03 -0.62

Bat Time Point Comparisons

bat 3h 7h 23h FC3h_7h FC3h_23h FC7h_23h
Mock 2814.63 2848.42 2887.49 0.02 0.04 0.02
Ebov 3248.88 3287.36 4515.23 0.02 0.47 0.46
Marv 2635.18 3225.69 2947.89 0.29 0.16 -0.13