WWP1

NameSynonymsFull NameRefSeq IDDescription (.pdf)IGV-img (humangenome)Sashimi-img (humangenome)UCSC-img (humangenome)IGV-img (batgenome)
WWP1 NM_007013, NP_008944, O00307, Q5YLC1, Q96BP4, Q9H0M0, WWP1_HUMAN,AIP5, DKFZP434D2111 WW domain containing E3 ubiquitin protein ligase 1 NM_007013 pdf

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Description

Homo sapiens WW domain containing E3 ubiquitin protein ligase 1 (WWP1) are WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined. This gene is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF.

The WWP1 gene shows a minor upregulation in human cells without infection and in the Marburg samples. Instead the Ebola infected human cells tissue shows a two fold upregulation, followed by a downregulation after 7 h. The mapping results to the homolog annotation in bat cells transcriptome does not show any differential expression in-between the 3-7-23 h samples. The mapping results also shows a 5000\,nt too long annotation at 3' end. The included exon is already the first on which belongs to the downstream located RMDN1 gene.

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Maximum read counts and DESeq normalized read counts for human and bat cell lines

Source Species Mapping on Mock3h Mock7h Mock23h EBOV3h EBOV7h EBOV23h MARV3h MARV7h MARV23h
Read_Max
humanH. sapiensGenome225 230 278 187 315 236 253 253 280
Read_Max
batR. aegyptiacusTranscriptome 55 55 42 37 33 51 47 45 50
Read_Max
bat_genomeR. aegyptiacusGenome 138 122 57 93 68 55 69 148 114
DESeq
humanH. sapiensGenome 3744.55 3671.52 4679.19 3535.98 4165.75 3072.36 3455.43 3650.83 5239.84
DESeq
batR. aegyptiacusTranscriptome 226.16 240.63 206.22 178.64 184.98 231.97 254.63 178.63 188.19
DESeq
bat_genomeR. aegyptiacusGenome 2530.9 2546.39 2298.5 2429.21 2365.09 2347.13 2376.14 2354.26 2264.63

Human Condition Comparisons

human Mock EBOV MARV FCMock_EBOV FCMock_MARV FCEBOV_MARV
3h 3744.55 3535.98 3455.43 -0.08 -0.12 -0.03
7h 3671.52 4165.75 3650.83 0.18 -0.01 -0.19
23h 4679.19 3072.36 5239.84 -0.61 0.16 0.77

Human Time Point Comparisons

human 3h 7h 23h FC3h_7h FC3h_23h FC7h_23h
Mock 3744.55 3671.52 4679.19 -0.03 0.32 0.35
Ebov 3535.98 4165.75 3072.36 0.24 -0.2 -0.44
Marv 3455.43 3650.83 5239.84 0.08 0.6 0.52

Bat Condition Comparisons

bat Mock EBOV MARV FCMock_EBOV FCMock_MARV FCEBOV_MARV
3h 226.16 178.64 254.63 -0.34 0.17 0.51
7h 240.63 184.98 178.63 -0.38 -0.43 -0.05
23h 206.22 231.97 188.19 0.17 -0.13 -0.3

Bat Time Point Comparisons

bat 3h 7h 23h FC3h_7h FC3h_23h FC7h_23h
Mock 226.16 240.63 206.22 0.09 -0.13 -0.22
Ebov 178.64 184.98 231.97 0.05 0.38 0.33
Marv 254.63 178.63 188.19 -0.51 -0.44 0.08