Differential transcriptional responses to Ebola and Marburg virus infection in cells from bats and humans

Supplemental Material

TPCN2

Name	Synonyms	Full Name	RefSeq ID	Description (.pdf)	IGV-img (humangenome)	Sashimi-img (humangenome)	UCSC-img (humangenome)	IGV-img (batgenome)
TPCN2	FLJ41094, SHEP10, TPC2,TPC2	two pore segment channel 2	NM_139075	pdf			NONE

Download all snapshots for IGV, UCSC and Sashimi (zip archive)

Description

This gene encodes a putative cation-selective ion channel with two repeats of a six-transmembrane-domain. The protein localizes to lysosomal membranes and enables nicotinic acid adenine dinucleotide phosphate (NAADP) -induced calcium ion release from lysosome-related stores. This ubiquitously expressed gene has elevated expression in liver and kidney. No differential expression changes were observed within human and bat cell lines between the different time points and infection states. Nevertheless, the ion channel encoded by this gene was recently reported as an important entry point for EBOV (Falzarano and Feldmann, February 2015, Science Virology). In the \textttDESeq expression data an overall higher expression of TPCN2 could be observed compared to the bat cell line, which could be one reason for the slower replication start of the filoviruses in bats compared to human.

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Read_Max

R. aegyptiacusGenome 33 32 25 24 19 22 14 43 36

Maximum read counts and DESeq normalized read counts for human and bat cell lines

Source		Species	Mapping on	Mock3h	Mock7h	Mock23h	EBOV3h	EBOV7h	EBOV23h	MARV3h	MARV7h	MARV23h
Read_Max		H. sapiens	Genome	43	69	64	59	73	73	66	92	41
Read_Max		R. aegyptiacus	Transcriptome	24	23	29	25	16	27	23	30	28
DESeq		H. sapiens	Genome	1288.76	1356.41	1466.41	1375.52	1482.2	1310.34	1565.06	1610.09	1141.63
DESeq		R. aegyptiacus	Transcriptome	81.65	78.15	124.38	98.98	75.47	135.73	93.33	110.89	112.09
DESeq		R. aegyptiacus	Genome	362.66	382.41	494.29	355.49	353.81	439.92	278.88	451.98	481.18

Human Condition Comparisons

	Mock	EBOV	MARV	FCMock_EBOV	FCMock_MARV	FCEBOV_MARV
3h	1288.76	1375.52	1565.06	0.09	0.28	0.19
7h	1356.41	1482.2	1610.09	0.13	0.25	0.12
23h	1466.41	1310.34	1141.63	-0.16	-0.36	-0.2

Human Time Point Comparisons

	3h	7h	23h	FC3h_7h	FC3h_23h	FC7h_23h
Mock	1288.76	1356.41	1466.41	0.07	0.19	0.11
Ebov	1375.52	1482.2	1310.34	0.11	-0.07	-0.18
Marv	1565.06	1610.09	1141.63	0.04	-0.46	-0.5

Bat Condition Comparisons

	Mock	EBOV	MARV	FCMock_EBOV	FCMock_MARV	FCEBOV_MARV
3h	81.65	98.98	93.33	0.28	0.19	-0.08
7h	78.15	75.47	110.89	-0.05	0.5	0.56
23h	124.38	135.73	112.09	0.13	-0.15	-0.28

Bat Time Point Comparisons

	3h	7h	23h	FC3h_7h	FC3h_23h	FC7h_23h
Mock	81.65	78.15	124.38	-0.06	0.61	0.67
Ebov	98.98	75.47	135.73	-0.39	0.46	0.85
Marv	93.33	110.89	112.09	0.25	0.26	0.02